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Circulation and distribution of 111-In-oxine-labeled autologous baboon platelet aggregates and buffy coat.

Valeri CR, Macgregor H, Giorgio A, Ragno G

Naval Blood Research Laboratory, Boston University School of Medicine, 615 Albany Street, Boston, MA 02118, USA. navblood@nbrl.org

BACKGROUND: Although it is known that RBC concentrates may contain buffy coat and platelet concentrates may contain platelet aggregates, the circulation and distribution of these materials in the blood products have never been reported. STUDY DESIGN AND METHODS: Baboon platelets were labeled with 111-In-oxine, aggregated with ADP and autotransfused without a filter. Baboon buffy coat was stored at 4 degrees C, labeled with 111-In-oxine and autotransfused without a filter. The circulation of the radiolabeled platelets and buffy coat was measured and the distribution of the buffy coat and platelet aggregates was measured by external scanning of the baboon using a gamma camera. The effects of the infusion of aggregated platelets, buffy coat, and gelatin on the plasma fibronectin level also were evaluated. RESULTS: The 111-In-oxine labeled platelet aggregates were initially sequestered in the lungs and released into the peripheral blood during the next 3h, during which time the cell associated radioactivity increased by about 25%. Following the autotransfusion of 111-In-oxine labeled buffy coat, the 111-In-oxine radioactivity over the lungs increased, but decreased during the 60-min post-transfusion period as the radioactivity over the liver increased. Cell-associated radioactivity increased by about 10% over the 3-h post-transfusion period. Fibronectin levels decreased by 3% following the autotransfusion of platelet aggregates, by 10% after the autotransfusion of buffy coat and by 50% after the infusion of gelatin. CONCLUSIONS: 111-In-oxine radioactivity in the platelet aggregates and buffy coat was initially sequestered in the lungs, and 10-25% of the 111-In-oxine cell-associated radioactivity was released into the circulation during the 24-h post-transfusion period.

Published 23 March 2005 in Transfus Apher Sci, 32(2): 139-46.
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